尖锐湿疣表皮CD1a和CD207的表达

Expression of CD1a and CD207 in condyloma acuminatum epidermis

目的:了解尖锐湿疣(CA)表皮中CD1a和CD207的表达情况及其意义。方法:采用寡核苷酸芯片对6例CA表皮和6例正常表皮中的CD1a和CD207的mRNA表达进行检测,应用半定量RT-PCR验证CD1a和CD207的mRNA表达,采用Western blot法检测6例CA表皮和6例正常表皮中的CD1a和CD207的蛋白表达。结果:基因芯片检测到CD1a和CD207的mRNA在CA表皮中表达下调,半定量RT-PCR证实CD1a和CD207的mRNA在CA表皮中表达下调,West-ern blot法检测到CD1a和CD207蛋白在CA表皮中的显著下调表达。结论:CA表皮中CD1a和CD207的表达下调,提示CA表皮中LC可能出现了数目的减少及功能的受损。

AIM： To investigate the expression of CDla and CD207 in condyloma acuminatum （CA） epidermis and to understand its significance. METHODS： The mRNA expression of CDla and CD207 in six CA epidermal lesions and in six normal controls were detected using oligonucleotide microarrys and confirmed by semi-quantitative RT-PCR, and the protein level of CD1a and CD207 in six CA epidermal lesions and in six normal controls were measured by Western blot. RESULTS： With microarrys, the mRNA expression of CD1a and CD207 were detected markedly downregulated in six CA epidermal lesions as compared with that in six normal controls. Moreover, the down-regulation of CD1a and CD207 was verified by semi-quantitative RT-PCR. Western blot analysis showed that the protein expression of CD1a and CD207 in six CA epidermal lesions were significantly lower than that in normal controls. CONCLUSION： The expression of CDla and CD207 is markedly down-regulated in CA epidermis compared with that in normal epidermis, and the results may suggest that the number of LC in CA epidermis is decreased and the function is impaired.