CyPA对类风湿关节炎中性粒细胞的趋化和产生IL-8的作用及其意义

The role of CyPA in chemotaxis of neutrophil in rheumatoid arthritis and secretion of interleukin-8

目的:观察细胞环亲素A(CyPA)对人中性粒细胞HL-60株和类风湿关节炎(RA)患者外周血中性粒细胞的趋化和产生IL-8的作用。方法:选择12例RA患者,分离外周血中性粒细胞,采用Boyden小室趋化实验比较CyPA和/或IL-8对HL-60和RA患者粒细胞的趋化作用,采用ELISA检测CyPA促进培养的粒细胞产生IL-8作用,并与健康人比较。结果:CyPA组、IL-8组及CyPA+IL-8组较阴性对照组趋化性显著增强(P<0.05);与IL-8组相比,CyPA抗体+IL-8组趋化指数降低(P<0.05);与CyPA组或IL-8组相比,CyPA+IL-8组趋化指数有所增加。RA患者中性粒细胞培养上清中IL-8水平较健康人显著增加(P<0.05);经CyPA刺激后,RA患者中性粒细胞培养上清中IL-8水平较CyPA刺激前增加,差异有统计学意义(P<0.05);阻断CyPA后,RA患者中性粒细胞培养上清中IL-8水平较CyPA刺激前无明显差异。结论:CyPA影响IL-8对中性粒细胞的趋化作用并对其分泌有影响。

AIM： To ascertain the effect of CyPA and IL-8 in chemotaxis of neutrophil and the level of IL-8 from the CyPA effecting of peripheral blood from RA patients. METHODS： 12 RA patients matched 4 healthy people were studied. Chemotaxis of IL-8 was measured by Boyden chamber on neutrophil of RA patients and that of 4 normal healthy people controls were studied; the level of IL-8 on neutrophil of RA patients peripheral blood after the effecting of CyPA was assessed by ELISA. Correlations between CyPA and IL-8 were observed in RA. RESULTS： The chemotaxis of neutrophil which IL-8 mixed with CyPA antibody was lower than IL-8 （ P 〈 0.05 ） ; The secretion of RA peripheral blood in IL-8 was higher than normal people, as well as the secretion of RApedpheral blood in IL-8 after effecting of CyPA was higher than before （ P 〈 0.0.5 ）, but the level of IL-8 after blocking CyPA was not changed. CONCLUSION： CyPA could affect the chemotaxis of IL-8 in the neutrophil of RA patients＇ peripheral blood. The secretion of IL-8 is accelerated by CyPA on neutrophil of RA patients＇ peripheral blood.