高效纤维素降解菌系的构建

Construction of A Microbial System for Efficient Degradation of Cellulose

筛选出能产生不同纤维素酶的10株纤维素降解菌,系统地分析了各菌株的EG、CBH和BG酶等3类纤维素酶活.经各菌株优化组合、混合培养,构建了一组由5株细菌(LCB03、LCB12、LCB52、LCD12和LCD51)组成、能协同作用的复合微生物菌系.经生理生化和分子水平鉴定,这5株细菌分别为Pseudomonas citronellolis(香茅醇假单胞菌)、Stenotrophomonas malto-philia(嗜麦芽寡食单胞菌)、Pseudomonas aeruginosa(铜绿假单胞菌)、Pseudomonas aeruginosa(铜绿假单胞菌)和Flavobac-teriummizutaii(水氏黄杆菌).复合菌系的各菌株可产生不同类型的纤维素酶,且各类酶可以协同作用有效分解天然纤维素,在纤维素类污染的治理与资源化利用中具有很好的应用前景.

Endoglucanase, exoglucanase and cellobiase activities of ten cellulose degradation isolates from piggery sludge and manure were analyzed. A complex microbial system was constructed by five bacteria named as LCB03, LCB12, LCB52, LCD12 and LCD51 respectively with different cellobiase activities from these isolates. Tested from the perspective of physiology and mierochemistry as well as molecular level, these five bacteria were identified as Pseudomaonas citronellolis, Stenotrophomonas maltophilia, Pseudornonasaeruginosa, Pseudomonas aeruginosa and Flavobacteriummizutaii, respectively. So far, there are few papers on cellulose degradation using S. maltophilia and F. mizutaii. Each of the five bacteria could produce different types of cellobiase that co-catalyze degrading natural cellulose effectively, and the microbial system therefore has great potential in cellulose-related pollution control and waste utilization.