不同浓度的EGF对山羊毛囊干细胞体外培养的影响

Effect of Different Concentrations of EGF on the Culture of Goat Hair Follicle Stem Cells

采用2.4 U/mL Dispase酶消化和机械切割法分离毛囊隆突部,经胰酶(0.5 mg/mL胰酶+0.2 mg/mL EDTA)消化从山羊耳部皮肤分离得到毛囊干细胞,并检测4种不同浓度(0、10、15和20 ng/mL)的EGF对山羊毛囊干细胞体外培养的作用。结果表明,EGF对毛囊干细胞体外培养效果明显,最适EGF浓度为15ng/mL。

Hair follicles were isolated from goat ear skin by were separated with special fine needle and digested with O. The resultant cells were cultured in four different concentr results showed that EGF has a good effect on the goat hair was best congcentration. 2.4U/mL Dispase digestion and the bulges 5 mg/mL trypsin plus 0.2 mg/mL EDTA. ations of EGF（0,10 ,15 ,20 ng/mL）. The follicle stem cells in vitro and thel5 ng/mL