皮下注射的骨髓间充质干细胞在正常小鼠体内的迁移分化

The migration and differentiation of bone marrow mesenchymal stem cells in normal mice in vivo

目的:探讨骨髓间充质干细胞(BM-MSCs)在正常小鼠体内从皮下向不同器官迁移的能力。方法:从β半乳糖苷酶(β-gal+)转基因小鼠分离骨髓细胞,经贴壁和传代培养获得第3代β-gal+BM-MSCs,皮下注射给正常6周龄C57BL/6J野生型小鼠。8周后通过LacZ染色示踪β-gal+BM-MSCs在受体小鼠不同组织器官的分布,并对供体第3代β-gal+BM-MSCs基质金属蛋白酶(MMPs)的基因表达水平进行检测。结果:β-gal转基因小鼠来源的供体第3代BM-MSCs呈现LacZ染色阳性。第3代的β-gal阳性BM-MSCs皮下注射8周后,在受体小鼠肺、脑、心、肝、肾内检测到LacZ染色阳性的细胞。第3代β-gal+BM-MSCs表达MMPs基因。结论:皮下注射的BM-MSCs能够在正常小鼠体内经血液循环迁移到不同器官,并可能分化成为不同器官的终末分化细胞,参与正常组织的自我更新。

Objective：To determine whether bone marrow mesenchymal stem cells （BM-MSCs） can migrate subcutaneously into different organs. Methods：The bone marrow cells isolated from β-galactosidase transgenic mice were cultured. BM-MSCs were passaged until the 3rd generation. The 3rd passaged BM-MSCs derived from β-galactosidase transgenic mice were transplanted into 6-week-old C57BL/6J wild type mice subcutaneously. 8 weeks later, β-gal^＋ MSCs were shown the distribution in various organs from recipient mice through LacZ staining. The expression of matrix metalloproteinase （MMPs） genes in the 3rd passaged β-gal^＋ BM-MSCs were examined by real-time RT-PCR. Results：The 3rd passaged BM-MSCs derived from β-galaetosidase transgenic mice were positive for LacZ staining. Followed subcutaneous injection of the 3rd passaged β-gal^＋ BM-MSCs for 8 weeks,β-gal positive organ specific cells were detected in lung,brain,heart,liver and kidney from recipient mice. MMPs genes were expressed in the 3rd passaged β-gal^＋ BM- MSCs. Conclusion： BM-MSCs transplanted through subcutaneously can migrate into various organs through blood circulation and may differentiate into various organ specific cells to participate in self-renewal of normal tissues.