摘要
目的:探讨Rac1、HIF-1αmRNA和蛋白在缺氧的人食管鳞癌Eca109细胞株中的表达及其对细胞增殖水平的影响。方法:应用半定量RT-PCR、Westernblot法检测缺氧条件下Eca109细胞株Rac1、HIF-1α的mRNA和蛋白表达水平;应用半定量RT-PCR和MTT法检测常氧和缺氧下Rac1特异性抑制剂NSC23766处理后人食管鳞癌Eca109细胞株HIF-1α的mRNA表达水平和细胞增殖水平。结果:缺氧条件下,人食管鳞癌Eca109细胞株中Rac1、HIF-1α的mRNA和蛋白表达水平均明显增高,约24h到达高峰。用NSC23766处理人食管鳞癌Eca109细胞株后,HIF-1ɑmRNA表达水平降低,细胞增殖明显受到抑制。结论:Rac1和HIF-1α在缺氧状态下被激活,且可能均与缺氧诱导的肿瘤增殖相关。
Objective:To investigate the mRNA and protein expressions of Rac1 and HIF-1α in human esophageal squamous cell cancer Eca109 and the effects on the cell proliferation of Eca109. Methods:The mRNA and protein expressions of Rac1 and HIF-1α were detected by semi-quantitative RT-PCR and Western blot under hypoxia;The expression of HIF-1α mRNA and the cell proliferation were detected by semi-quantitative RT-PCR and MTT in Eca109 cell line with the use of Rac1 specific inhibitor NSC23766 under normoxia and hypoxia. Results:The mRNA and protein expressions of Rac1 and HIF-1α were upregulated in Eca109 cell line by hypoxia,up to the maximum in 24 h. Rac1 specific inhibitor NSC23766 reversed the hyoxia-induced upregulation of HIF-1α mRNA and significantly inhibited the proliferation of Eca109 cell line. Conclusion: Rac1 and HIF-1α were both activated by hypoxia,and might be involved in the proliferation of the cancer cell.
出处
《南京医科大学学报(自然科学版)》
CAS
CSCD
北大核心
2009年第5期652-655,710,共5页
Journal of Nanjing Medical University(Natural Sciences)