前列腺素E1、腺苷对离体兔肺保护作用良好

The protective effect of PGE and adenosine perfsuion on the isolated lung of rabbit

目的:研究前列腺素E1以及腺苷对离体兔肺的保护作用。方法:将18只新西兰大白兔分为3个实验组,分别为单纯UW液(A组、对照组)、UW液+前列腺素E1(B组)、UW液+腺苷(C组)。切取实验兔整体心肺作为离体心肺模型,对心肺模型进行冷灌注、低温保存。分别在2h末和4h末,取肺部分标本做电镜分析。于4h末,结扎右肺门,取右肺检测肺湿/干重比(W/D)、测肺气道压力(Paw)。左侧离体心肺接呼吸机模拟移植手术后呼吸,并将自身静脉血用泵从主肺动脉泵入,用负压吸引真空试管取左心房血液做血气分析。结果:B组肺组织气体交换功能的保护作用以及降低供体肺保存过程中含水量的作用明显优于其他组;降低气道压力和C组相当,明显优于对照组。C组肺实质细胞的气体交换功能的保护、降低肺含水量以及降低气道压力明显优于A组。结论:UW液中加入前列腺素E1进行离体肺灌注可以使肺保存时间明显延长、肺气体交换功能保存良好。

Objective：To investigate the protective effect of PEG1 and adenosine perfusion on the isolated lung of rabbit. Methods： Eighteen rabbits were randomly divided into 3 groups which contain control group（A）,UW fluid with PGE1 group（B）,UW fluid with adenosine group （C）. The lungs and hearts of rabbits were dissociated as the isolated lung and heart model. The model was perfused with low temperature perfusion fluid and conserved. The lung specimens were obtained in order to analyze the changes in morphology by using electron microscope at 2 and 4 h after perfusion. The ratio of wet/dry weight（W/D）, airway pressure（Paw）of lung model, gas analysis of the blood which was pumped into the lung model through main pulmonary artery were detected, and then the blood which was elicited from left atrium pulmonale by using a vacuum aspiration tube after 4 hours of perfusion was collected. Results：The protective effects on the gas exchange function of the lung and the moisture capacity in group B were better than those in group A and C significantly. The protective effect on Paw, moisture capacity and the gas exchangs function of lung of group C was better than those of group A. Conclusion：Perfusion of the isolated lung with PGE1 can get longer time of preservation and better function of lung tissue gas exchange than pertusion with UW solution.