摘要
目的:探讨蟾蜍灵(bufalin)对脂多糖(lipopolysaccharides,LPS)诱导的大鼠肾小球系膜细胞(glomerular mesangial cell,GMC)凋亡的影响。方法:体外培养大鼠肾小球系膜细胞,分为对照组、LPS刺激组和蟾蜍灵干预组,应用台盼蓝拒染法检测蟾蜍灵对GMC的细胞毒性作用,应用透射电镜观察系膜细胞超微结构的变化,采用逆转录PCR检测Bax和Bcl-2基因mRNA的表达,Western blot法检测Bax和Bcl-2的蛋白表达。结果:透射电镜观察蟾蜍灵干预组可见凋亡早期形态学改变。逆转录PCR和Western blot结果显示蟾蜍灵组较脂多糖组Bax表达增多,Bcl-2表达减少,差异均具有统计学意义(P<0.01),蟾蜍灵干预后,Bax较Bcl-2表达过量。结论:蟾蜍灵可能通过调节Bax和Bcl-2的相对表达量而诱导脂多糖作用后肾小球系膜细胞发生凋亡。
Objective:To investigate the effects of bufalin on glomerular mesangial cells (GMC)apoptosis induced by lipopolysaccharide (LPS). Methods: Rat GMC lines were cultivated in vitro and divided into 3 groups:control group, LPS group (LPS 5mg/L), bufalin group(LPS 5 mg/L and bufalin 1×10^-8 mol/L).Cell structure was observed by electron microscrop.The transcriptional level of Bax and Bcl-2 gene were detected by RT-PCR. The protein expression level of Bax and Bcl-2 were detected by Western blot method. Results: The morphology of GMC presented changes of apoptosis with electron microscopg. The mRNA and protein expression of Bax in bufalin group were more significant than those in LPS group (P 〈 0.01), but the mRNA and protein expression of Bcl-2 were less than those in LPS group(P 〈 0.01). Conclusion:Bufalin may induce the cell apoptosis of lipopolysaccharide-induced GMC by modulating Bax and Bcl-2.
出处
《南京医科大学学报(自然科学版)》
CAS
CSCD
北大核心
2009年第5期674-677,714,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
南京医科大学科研发展基金(07NMUM044)