阿糖胞苷对白血病细胞K562凋亡抑制基因Survivin的作用研究

Studies on the expression of Survivin in human leukemia K562 treated by cytarabine

目的了解人类白血病细胞在化疗药物阿糖胞苷作用下的Survivin凋亡抑制基因表达情况，探讨阿糖胞苷治疗耐药的可能机制。方法用MTT比色法检测K562细胞对阿糖胞苷的敏感性，以阿糖胞苷的，c钔药物浓度处理K562细胞。应用RT—PCR和Western blotting的方法检测各组细胞Survivin基因的mRNA表达情况和蛋白质的表达情况。结果K562细胞经阿糖胞苷作用24、48h后，细胞的Survivin mRNA表达较加药前分别增加1．92、3．38倍（P〈0．05）；24、48h阿糖胞苷作用组细胞的Survivin的蛋白表达较对照组分别增加1．92、2．64倍（P〈0．05）。结论阿糖胞苷作用白血病细胞后，细胞的Survivin基因mRNA和蛋白质表达升高，且具有时间效应。化疗药物作用后Survivin基因表达升高可能是自血病细胞治疗耐药的又一机制。

Objective To investigate the influences of cytarabine on Survivin gene expression in human leukemia K562 cell line and discuss the mechanism of drug resistance in chemotherapy. Methods The ICso of cytarabine was chosen by MTT assay. The K562 cells were exposed to certain concentration of cytarabine for about 24 hours and 48 hours. The expression levels of Survivin gene were detected by RT-PCR and Western-blot. Results After exposed to cytarabine for 24 hours and 48 hours, the Survivin mRNA level of K562 cells was significantly elevated about 1.92 and 3.38-fold, and the protein expression level was elevated about 1.92 and 2.64-fold. Conclusion An elevated expression of Survivin was tested in K562 cells treated by cytarabine. Consequently, the elevated expression of Survivin could resist apoptosis induced by chemotherapeutic agent which probably associated with chemotherapeutic drug resistance.