多参数流式细胞术在成年人急性髓系白血病免疫分型中的应用

Study on immunophenotyping with multi-color flow cytometry in adult patients with acute myeloid leukemia

目的探讨成年人急性髓系白血病（AML）三色流式细胞术免疫分型的特点。方法采用CD45／SSC双参数散点图设门方法对126例成年AML患者进行三色流式细胞术免疫分型分析。结果髓系抗原CD13、CD33和CD117在AML各亚型中均有很高的表达，阳性率分别为86．4％、70．2％和90．4％；CD34及HLA—DR表达偏低，分别为63．5％和61．7％。约34．2％AML患者伴有淋系抗原表达，AML淋系抗原CD7和CD19表达率分别为23．6％和2．3％。结论流式细胞术免疫分型对AML的诊断及预后判断具有重要的意义。

Objective To evaluate the characteristics of immunophenotyping in adults with acute myeloid leukemia （AML） using multi-color flow cytometry. Methods Immunophenotyping was performed by three color flow cytometry using CD45/SSC gating. Results In 126 patients with AML, the myeloid antigen of CD13, CD33 and CDH7 was highly expressed. The positive rate was 86.4 %, 70.2 % and 90.4 %, respectively. The CD34 and HLA-DR were lowerly expressed as 63.5 % and 61.7 %, respectively. About 34.2 % of lymphoid-associated antigen expression of all the AML patients. The lymphoid-associated antigens of CD7 and CD19 expression in patients with AML was 23.6 % and 2.3 %, respectively. Conclusion Multi-color flow cytometry is an important method for diagnosis and prognosis for AML.