胆盐(GCDA)对肝癌细胞凋亡及其药物敏感性的影响

Effects of bile salt(GCDA) on apoptosis and drug sensitivity of hepatocellular carcinoma cells

目的探索胆盐(GCDA)环境下肝癌细胞株HepG2的生长情况及其对抗肿瘤药物的敏感性。方法用不同时间或浓度GCDA处理HepG2细胞,采用MTT法检测细胞活性与增殖性;用3种抗肿瘤药对HepG2细胞进行处理,采用流式细胞分析法检测GCDA存在与缺少时的细胞周期与凋亡。结果GCDA的处理浓度在200μM内对细胞有明显增殖作用(P<0.05);GCDA的处理时间在4h内对细胞有增殖作用,以10～20min最明显(P<0.05)。3种药物对肝癌HepG2细胞均有杀伤作用,阿霉素(ADM)对细胞周期的影响阻滞在G0/G1,Oxaplatin与Irinotecan阻滞在S期,GCDA使Irinotecan作用下细胞凋亡率明显下降(P<0.05)。结论GCDA能诱导肝细胞癌细胞株HepG2细胞存活与增殖,GCDA对Irinotecan的药性有抵制作用,使肿瘤细胞对其的敏感性降低。

[Objective] To study the growth of HCC cell line HepG2 and its sensitivity to antitumor drugs under bile salt （GCDA） environment. [Methods] HepG2 cells were treated with different concentration of GCDA or treated for different time. Cell viability and proliferation were detected by MTT assay. Cells were treated with three antitumor drugs in presence or absence of GCDA. Cell cycle and apoptosis were assayed by Flow Cytometry （FCM）. [Resuits] GCDA has obvious effect on cell proliferation when the concentration is 200 μM （P 〈0.05） or the time is within 4 hours, especially 10 to 20 minutes （P 〈0.05）. Three antitumor drugs have killing effect on HepG2 ceils. ADM arrests the cell cycle in G0/G1 phase, while Oxapladn and Irinoteean arrest in S phase. GCDA causes Irinotecan-induced apoptotic rate decreased remarkably （P 〈0.05）. [Conclusions] GCDA can induce the HCC cell line HepG2 survival and proliferation. GCDA has resistant effect on drug property of Irinotecan and cause drug sensitivity decreased and chemoresistance of tumor cells.