O_2^(·-)损伤过氧化氢酶过程中辅基Fe(Ⅲ)的ESR波谱

ESR Spectra of Prosthetic Group Fe(Ⅲ) in Injuring Catalase with O ·- 2

在体外Ｏ·－２损伤ＣＡＴ的过程中，对ＣＡＴ辅基Ｆｅ（Ⅲ）的ＥＳＲ波谱进行了分析研究。发现：辅基Ｆｅ（Ⅲ）的ＥＳＲ谱线特征有显著改变，如：ｇ因子、ΔＨｐｐ及线型等均有显著变化。说明：辅基Ｆｅ（Ⅲ）的微观环境发生了变化，提示：这些变化可能是Ｏ·－２造成ＣＡＴ活性改变的主要分子机制。

We studied the ESR spectra of the prosthetic group Fe(Ⅲ) in injuring CAT with O ·- 2 in vitro. We found that the Fe(Ⅲ) ESR spectra had significant changes, such as the g-factor, ΔHpp and the curve type and so on. The results show that the Fe(Ⅲ) microenvironment had changed, which may be a main molecular mechanism of CAT activity change in injuring with O ·- 2.