虾夷扇贝线粒体非编码区2(NcR2)序列多态性研究

Sequence Analysis of Non-coding Region 2(NcR2) in mtDNA of Japanese Scallop(Patinopecten yessoensis)

利用PCR产物直接测序法对大连獐子岛(ZZD)、大连旅顺口(LSK)、日本青森县(QSX)及俄罗斯海森崴(HSW)4个群体40个虾夷扇贝的线粒体DNA序列变异进行分析。共测定了40个序列,得到14个单元型。除NcR2序列外,其他序列无变异存在。在NcR2序列中,有15个变异位点,包括9个转换位点、2个颠换位点、4个插入/缺失位点。ZZD群体含有的单元型最多,为6种,HSW群体含有5种,LSK群体和QSX群体各含有3种,大连的2个群体中均具有与日本群体相同的单元型H2,表明它们之间关系较近。ZZD群体的单元型多态性比例最高(60%),HSW次之,LSK与QSX群体最低,均为30%,说明獐子岛群体的遗传多样性较高。

Analysis of sequence variation in a mitochondrial DNA Non-coding Region 2 （NcR2） was conducted in 40 individuals of four populations of Japanese scallop （Patinopecten yessoensis）from Dalian （Zhangzhidao, ZZD and Lvsun,LSK）,Japan （Aomori,QSX）and Russia（Vladivostok, HSW）. Fifteen variable sites including nine transitions, two transversions and four indels were found from the NcR2 segment of 14 haplotypes, of which six were observed in ZZD, five in HSW, three in LSK and QSX populations. Haplotype H2 was shared by population ZZD, LSK and QSX, indicating that the scallop in Dalian had closed relationship with that in Japan. ZZD population showed the highest haplotypic polymorphism, and had higher genetic diversity than other population.