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低电导钙激活的钾通道(rSK3，rIK)抑制露蜂房蛋白1(NVP(1))抗细胞增殖作用 被引量：1

Small-conductance Ca^(2+) activated K^+ channel inhibits the effect of NVP(1) anti-proliferation on Hek-293 cells
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摘要 目的研究rSK3,rIK通道在细胞增殖中的作用。方法用凝胶层析和液相色谱从露蜂房水提物中分离一种纯蛋白,并检测此蛋白的抗细胞增殖作用;转染;Southern blot检测rSK3,rIK基因;细胞计数检测SK通道在细胞增殖中的作用。结果从露蜂房水提物中分离一种纯蛋白,分子量为6.6 ku,命名为NVP(1),能抑制Hek-293细胞增殖。Southern blot检测转基因rSK3,rIK成功。转rSK3,rIK基因后,能消减NVP(1)抗细胞增殖的作用。结论rSK3,rIK促进细胞增殖。 Aim To investigate the effect of rSK3 and rIK anti-NVP （1） on inhibiting the proliferation of Hek-293 cells. Methods The effect of rSK3 and rIK anti-NVP （ 1 ） on Hek-293 cell proliferation was detected by cell count assay. Transfection and southern-blot were used to detect the rSK3 and rIK gene. Resultsr SK3 and rIK gene could prompt proliferation and inhibit the effect of NVP （ 1 ）. Conclusions rSK3 and rIK channel can prompt proliferation of Hek-293. SK may be a novel target on cancer therapeutics.
作者 汪长东 陈鹏 闫旭 何光源
机构地区 华中科技大学生科院中英联合实验室
出处 《中国药理学通报》 CAS CSCD 北大核心 2009年第5期577-580,共4页 Chinese Pharmacological Bulletin
基金 国家重点基础研究发展计划(973计划)资助项目(No2002CB111302)
关键词 rsK3 rIK 细胞增殖 rSK3 rIK cell proliferation
分类号 R282.74 [医药卫生—中药学] R329.24 [医药卫生—人体解剖和组织胚胎学]
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参考文献7

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中国药理学通报
2009年 第5期

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