体内IL-2基因和IFN-γ基因转染巨噬细胞抗肿瘤作用的研究

The Antitumor Effects of Mice Peritoneal Macrophages Transferred IL-2 and IFN-Y Gene in vivo

将IL-2或/和IFN-γ基因体内转染腹腔巨噬细胞(MΦ),其表达的基因产物能提高MΦ的细胞毒活性,并诱导MΦ分泌TNF、IL-1和NO等抗肿瘤免疫介质.激发机体的非特异性免疫功能而产生抗肿瘤效应,特别是IL-2和IFN-γ基因联合转染MΦ,其表达的基因产物既能使MΦ产生更强的细胞毒活性并分泌高水平的TNF、IL-1和NO,又能激活脾CTL细胞,从而激发机体的非特异性和特异性免疫功能,产生更强的快同抗肿瘤效应.结果表明。

The eukaryotic expression vector pREP-8-IL-2 or and pREP-8-IFN-γ was injected i.p. into mice by calcium phosphate coprecipitation method and IL-2 gene or IFN-γ gene was successfully transfected into peritoneal Mφs whose expression products could enhance the cytotoxicity of Mφs, which secrete some TNF, IL-1 and NO, activate the nonspecific immunity and inhibit the growth of tumor effectively. In particular, IL-2 gene in combination with IFN-γ gene transfection were successfully transfected into peritoneal Mφs whose high expression products not only significantly enhance the Mφs cytotoxicity and made it secrete high level TNF, IL-1 and NO, but also activated the CTLs of spleen and initiated specific immunity and nonspecific immunity . This would produce synergic antitumor effects. The results showed that IL-2 and IFN-γ gene transfection produced more antitumor effects than IL-2 or IFN-γ gene transfection alone.