摘要
目的建立双黄消炎片中盐酸小檗碱的含量测定方法。方法采用高效液相色谱(HPLC)法,以Hypersil ODS C18柱为色谱柱(150mm×4.6mm,5μm),以磷酸盐缓冲液(1∶1的0.05mol/L磷酸二氢钾和0.05mol/L庚烷磺酸钠,含0.2%三乙胺,并用磷酸调pH至3.0)-乙腈(60∶40)为流动相,检测波长为345nm,流速为0.8mL/min,柱温为35℃。结果盐酸小檗碱质量浓度在10.7～171.2μg/mL范围内与峰面积线性关系良好(r=0.9997),平均加样回收率为99.49%,RSD=1.92%(n=6)。结论HPLC法简便可行、重复性好,可用于双黄消炎片中盐酸小檗碱的含量测定。
Objective To estabish a HPLC method for the determination of berberine hydrochloride in Shuanghuangxiaoyan Tablets. Methods The Hypersil ODS C18 column （150 mm ×4.6 mm, 5 μm） was used. The mobile phase consisted of acetonitrile-potassium dihydrogen phos- phate（40 ： 60）with the flow rate of 0.8 mL/min. The detection wavelength was 345 nm and the column temperature was 35 ℃.Results There was a good linearity in the range of 10.7-171.2 μg/mL（r=0.999 7）.The average recovery rate was 99.49% and RSD was 1.92% （n =6）. Conclusion The method is efficient and accurate with good repeatability. It can be used for the control of Sbuanghuangxiaoyan Tablets.
出处
《中国药业》
CAS
2009年第10期41-42,共2页
China Pharmaceuticals
