摘要
目的建立耳聋左慈丸中马钱苷的含量测定方法。方法采用高效液相色谱法,用十八烷基硅烷键合硅胶为固定相,以乙腈-甲醇-0.05%磷酸溶液(8∶4∶88)为流动相,检测波长为236nm,流速为1.0mL/min。结果方法精密度和稳定性均良好,马钱苷进样量线性范围是101.6～812.4ng,r=0.9999(n=5),平均回收率为99.8%,RSD=0.61%(n=9)。结论该方法适用于耳聋左慈丸中马钱苷的含量测定。
Objective To build up a method for the determination of loganin in Erlong Zuoci Pills. Methods Using the HPLC method with C18 Column, the mobile phase was a mixture of acetonitrile-metbanol-0. 05% phosphoric acid （8：4：88）, the detection wavelength was at 236 nm and the flow rate was 1.0 mL/min. Results The precision and stability were fine. The linear correlation was observed from 101.6- 812.4ng, r=0.9999（n=5）,The average recovery rate was 99.8%, RSD=0.61% （n=9）. Conclusion The method can be used to determine loganin concentration in Erlong Zuoci Pills.
出处
《中国药业》
CAS
2009年第10期42-43,共2页
China Pharmaceuticals
