己酮可可碱、孕酮和A23187对人精子甘露糖受体表达的影响

Effects of pentoxifylline, progesterone and A23187 on expression of mannoseligand receptor of human spermatozoa

人精子经ＢＷＷ获能５～６小时后，分别用己酮可可碱（ＰＦ）、孕酮（Ｐ）和Ａ２３１８７三种已知的获能及（或）顶体反应促进剂处理１小时，对实验和对照组精子用异硫氰酸荧光素标记的甘露糖化牛血清白蛋白（ＭＦＩＴＣＢＳＡ）作探针标记精子甘露糖受体（ＭＲ）。结果表明，获能促进剂ＰＦ并不促进ＭＲ表达而Ｐ和Ａ２３１８７则有显著促进ＭＲ表达的作用。Ｐ对ＭＲＩＩ型的促表达作用略强于对ＩＩ型，而Ａ２３１８７则对ＩＩ型的促进显著强于Ｉ型。认为ＭＲ的表达是一种依赖于钙离子的细胞生物学过程，并与顶体反应有密切关系。推测ＭＲ可能具有介导精子与卵膜融合的作用。

 Objectives: To examine the effect of pentoxifylline, progesterone and A 23187 on expression of mannoseligand receptor (MR) of human spermatozoa.Design: Experimental study performed with donor spermatozoa.Setting: Andrology laboratory in Fujian Medical University.Subjects: Donor semen from proven fertile man.Main outcome measure: Percentage of spermatozoa labeled with MFITCBSA.Methods: The swimmingup spermatozoa were incubated in capacitating medium BWW for 5～6 hours at 37℃ and then treated, respectively, with three kinds of known capacitation and/or acrosome reaction activators: progesterone, calcium ionophore A23187 and pentoxifylline. After an hour, the mannoseligand receptors(MRs) of experimental spermatozoa and their controls were probed with fluorescent isothiocyanatelabelled mannosylated albumin (MFITCBSA, Sigma A7790) and visuallized by fluorescence microscopy.Results: Progesterone and A23187 improved remarkably MRs expression, while pentoxifylline did not. Progesterone enhanced the MRs expression of pattern II, slightly stronger than of pattern III, whereas A23187 enhanced pattern III much stronger than pattern II. MRs expression of pattern II,were detected on the area of acrosome cap, while that of pattern III, the receptors concentrated as a bar like narrow bend at the equatorial segment and post acrosome area.Conclusion: According to the results we propose that MR might play an important role in intermediate human fertilization, especially in spermoocyte membrane fusion.