壳聚糖载药纳米微球对人肝癌细胞的杀伤作用及AFM探测

Probing the inhibitory effect of 5-Fu/Cs drug-loaded nanospheres to human hepatoma cells with atomic force microscopy

本文以5-氟尿嘧啶(5-Fu)为抗肿瘤药物模型,壳聚糖为载体,制备平均粒径约为250nm的5-氟尿嘧啶/壳聚糖(5-Fu/Cs)载药纳米微球。采用MTT法评价载药纳米微球在体外对人肝癌细胞BEL7402的杀伤率,并采用原子力显微镜探测用药处理前后的肿瘤细胞表面超微结构的变化。结果表明,载药纳米微球对肿瘤细胞的杀伤率在量效和时效上明显比同剂量单独5-Fu作用的杀伤率高;AFM结果显示5-Fu与载药纳米微球对人肝癌细胞BEL7402作用后的细胞膜表面超微结构的变化存在较大差异,载药纳米微球在肿瘤细胞表面的吸附作用或内吞作用,导致载药纳米微球在细胞表面的释药机制不同于单独药物的扩散模型。

5-Fluorouracil（5-Fu）/Chitosan（CS） drug-loaded nanospheres was prepared by ionic crosslinking method with tripolyphosphate （TPP） as crosslinker. The growth inhibitory of the 5-Fu/Cs drug-loaded nanospheres to human hepatocarcinoma cells BEL7402 was determined with the method of MTT. human hepatocarcinoma cell lines were sensitive to the nanospheres, and the cytoxidties were positive interrelated with reaction time and the logreleased dose. The morphoy of membrane surface uhrastructures of human hepatocarcinoma cell strain BEL7d02 were observed and compared by atomic force microscopy. And the effect of 5-Fu and 5-Fu/Cs drug-loaded nanospheres on the membrane surface of BEL7402 cell were studied. We found that there are distinct differences of membrane surface uhrastrures among drug-administrated ones.