脑源性神经营养因子在人牙髓干细胞中的表达及其对细胞增殖分化的影响

The mRNA expression of BDNF in human dental pulp stem cells and its effect on proliferation and differentiation of the cells

目的:检测脑源性神经营养因子(brain-derived neurtrophic factor,BDNF)在人牙髓干细胞中的表达,初步观察其对细胞增殖和分化的影响。方法:采用酶消化法分离培养人牙髓干细胞;RT-PCR检测细胞中BDNF mRNA表达;MTT和碱性磷酸酶活性检测观察其对细胞增殖分化的影响。结果:RT-PCR反应扩增出BDNF mRNA阳性产物;与对照组相比,25ng/mL BDNF可显著促进牙髓干细胞的增殖(P<0.05);100ng/mL时可显著提高碱性磷酸酶活性(P<0.05)。结论:BDNF可表达于人牙髓干细胞,对细胞增殖分化有一定促进作用。

AIM： To examine the expression of BDNF in human dental pulp stem cells（hDPSC） and the effect of BDNF on the proliferation and differentiation of human dental pulp stem cells in vitro. METHODS ： Dental stem cells were isolated and cultured by a digestion method, and MTT colorimetric method was used to detect the close -effect of BDNF on the proliferation of hDPSCs. The differentiation effect was evaluated by alkaline phosphatase （ALP） activity assay. RESULTS： When the experiment group was compared with the control group, BDNF of 25ng/ mL stimulated the cell proliferation significantly （P 〈 0.05） and the ALP activities were obviously increased with BD- NF of 100 ng/mL（P 〈 0.05）. CONCLUSION： hDPSCs can express BDNF mRNA while BDNF may play a role on the proliferation and differentiation of hDPSCs.