重度牙周炎病人非刺激性全唾液中IL-6和TNF-α检测

Expression of IL-6 and TNF-α activities in saliva of chronic periodontitic patients

目的:研究重度牙周炎病人非刺激性全唾液中白细胞-6(IL-6)和肿瘤坏死因子-α(TNF-α)活性与表达。方法:采集32例重度牙周炎病人和24例牙周健康者非刺激性全唾液,用ELISA法检测其中IL-6和TGF-α的含量,由1名牙周科医师检查记录相关临床指标,对所得结果进行统计学分析。结果:IL-6和TNF-α在重度牙周炎病人唾液中的含量分别为3945.01±611.23和5945.01±611.23,明显高于正常对照组1904.79±503.12和2104.79±503.12。结论:IL-6和TNF-α在重度牙周炎病人中表达水平明显增高,唾液中的IL-6和TNF-α水平有可能成为牙周炎诊断的分子标志物。

AIM：To detect the IL -6 and TGF -α activities in saliva of chronic periodontitic patients. METHODS： We selected 32 chronic periodontitic patients and 24individuals with healthy periodontium, collected their saliva without stimulation, detected the content of IL - 6 and TGF -α by using double sandwich antibody method, and con＇elated the results with clinical parameters by statistical analysis. RESULTS ： The contents of IL - 6 and TGF -α in saliva of chronic periodontitic patients were （ 3945.01±611.23 ） and （5945.01± 611.23 ）, obviously higher than those in the （1904.79±503.12） and （2104.79±503.12）. CONCLUSION：The level of IL -6 and TGF -α in saliva may be a molecular marker for the diagnosis of chronic periodontitis.