鱼肝微粒体苯并芘单加氧酶指示水体中多环芳烃污染的研究

STUDY OF BENZO (α) PYRENE MONO-OXYGENASA ACTIVITY OF FISH LIVER MICROSOMAL AS AN INDUCTOR FOR WATER POLLUTION BY POLYCYCLIC AROMATIC HYDROCARBONS

用苯并芘作为鲫鱼肝微粒体苯并芘单加氧酶(BPMO)的底物并采用Ⅱ号柴油作污染源,以0.01ppm,0.06ppm和30ppm三种不同浓度致毒鲫鱼。研究结果表明,三种浓度的污染源皆能诱导苯并芘单加氧酶活性且BPMO对底物有很高的灵敏度,显示出较高的酶活性。同时,BPMO活性随多环芳烃污染浓度的增加而增大。

Benzo(α)pyrene and Naphthalene were used as substrates for crucian carpliver microsomal preparation, and 0.017ppm, 0.06ppm and30ppm Diesel 2 oilwere used separately on crucian carp for 60 days. The result indicate thatthe three concentrations were shown to person significant on Benzo(α)pyreneMonoxygenase (BPMO) activity, and that the crucian carp microsomalpreparation had a hight affinity for substrates and the high BPMO activitywas depressed. It was shown an direct ratio relationship between BPMOactivity and the concentration of polycyclic aromatic hydrocarbon in water.