60 kD人SSA抗原表位免疫反应的特异性

IMMUNOLOGIC RESPONSE SPECIFICITY OF HUMAN 60 kD SSA AUTOANTIGEN EPITOPES

目的：根据６０ｋＤＳＳＡ抗原分子不同表位的氨基酸序列，人工合成多倍体寡肽抗原表位（ｍｕｌｔｉｐｌｅａｎｔｉｇｅｎｉｃｐｅｐｔｉｄｅｓ，ＭＡＰｓ），以检测这些表位的免疫特性。方法：用ＭＡＰｓ做亲和层析柱，用抗ＳＳＡ抗体的病人血清过柱，纯化抗ＭＡＰｓ抗体。这些纯化后的抗ＭＡＰｓ抗体可与６０ｋＤＳＳＡ抗原及ＭＡＰｓ反应。这种反应能被６０ｋＤＳＳＡ抗原及相对应的ＭＡＰｓ抑制。以ＭＡＰｓ做抗原检测６７份具有抗ＳＳＡ抗体的血清。结果：６７份病人血清中，６３份阳性，阳性率９４％，这些血清结合的ＭＡＰｓ不同，可以从１至２０个ＭＡＰｓ不等。结论：研究表明，６０ｋＤＳＳＡＭＡＰｓ保留了６０ｋＤＳＳＡ抗原的抗原性。

Objective:According to the different 60 kD human SSA antigenic polypeptide amino acid sepuences multiple antigenic peptides (MAPs) were synthesized to test the immunity of these antigenic epitope.Methods:AntiMAPs antibodies were purified by passing patient sera with antiSSA antibodies through affinity chromatography columns that were linked with selected SSAMAPs.These purified antibodies could respond to 60 kD human SSA antigen and relevant SSAMAPs.Sera with antiSSA antibodies of 67 patients were tested.Results:Of 67 patient sera,63 (94%) could bind to SSAMAPs and they could bind epitopes 1 to 20.Conclusion:It indicates that 60 kD human SSAMAPs keep the native molecular antigenicity.