类风湿关节炎中转化生长因子β_1和细胞间粘附分子的变化

CHANGES OF TRANSFORMING GROWTH FACTORβ1 AND INTRACELLULAR ADHESSIONMOLECULE IN RHEUMATOID ARTHRITIS

目的：了解类风湿关节炎（ＲＡ）血清、滑液和滑膜组织中转化生长因β１（ＴＧＦβ１）、细胞间粘附分子１（ＩＣＡＭ１）及细胞间粘附分子３（ＩＣＡＭ３）的变化。方法：采用ＥＬＩＳＡ及ＡＢＣ免疫组化方法检测ＲＡ患者血清、滑液和滑膜中ＴＧＦβ１、ＩＣＡＭ１和ＩＣＡＭ３的浓度和阳性程度。结果：ＲＡ血清中ＴＧＦβ１含量较低，而ＩＣＡＭ１含量明显升高，ＩＣＡＭ３含量正常，滑液中ＩＣＡＭ３含量低于血清含量。ＲＡ血清中ＴＧＦβ１含量与ＩＣＡＭ１含量呈中度负相关，与ＩＣＡＭ３含量无显著相关。在ＲＡ滑膜中巨噬细胞、滑膜衬里细胞和成纤维细胞ＴＧＦβ１染色阳性，巨噬细胞、滑膜衬里细胞、成纤维细胞和血管内皮细胞ＩＣＡＭ１染色阳性。结论：ＴＧＦβ１和ＩＣＡＭ１参与了ＲＡ的发生和发展过程，在ＲＡ慢性炎症中，ＩＣＡＭ１对炎细胞转移并聚集于滑膜可能有重要作用，ＲＡ外周血ＴＧＦβ１浓度减低伴随ＩＣＡＭ１浓度的增加。

Objective:To explore the change of transforming growth factorβ1 or intracellular adhession molecule in sera,synovial fluid and synovial tissue in patients with rheumatoid arthritis.Methods:The expression of TGFβ1,ICAM1 and ICAM3 in sera,synovial fluid or synovial tissue in patients with RA was measured or stained using ELISA method and avidinbiotinperooxidase technique.Results:The contents of serum TGFβ1 in patients with RA were lower than those in normal subjects.But the contents of serum ICAM1 in patients with RA were higher.The contents of serum ICAM3 in RA were similar to those of ICAM3 in normal subjects,but the contents of ICAM3 in the synovial fluid in patients with RA were lower than the contents of ICAM3 in the sera in patients with RA.The contents of serum TGFβ1 in patients with RA were negatively correlated with the contents of serum ICAM1 in patients with RA.Positive staining for TGFβ1 was found on macrophage,fibroblast and synovial lining cell in rheumatoid synovium.The staining of lymphocyte and endotheliocyte was negative in rheumatoid synovium.The staining of ICAM1 was positive on macrophage,synovial lining cell,fibroblast and endotheliocyte in rheumatiod synovium.Conclusion:TGFβ1 and ICAM1 take part in the occurrence or development course of RA.ICAM1 plays an important role in inflammatory cell transforming and aggregating to synovial tissue.The higher the contents of serum TGFβ1 in the patients with RA,the lower the contents of the sreum ICAM1.