卡泊芬净、米卡芬净对8种皮肤癣菌体外抑菌活性的研究

In vitro antifungal activity of caspofungin and micafungin against 8 species of dermatophyte

目的评价棘白菌素类抗真菌药物卡泊芬净(caspofungin)、米卡芬净(micafungin)针对常见致病性皮肤癣菌的体外抗菌活性。方法参考CLSI制定的M38-A2方案。测定82株常见皮肤癣菌的最低有效浓度(minimal effective concentrations,MECs)。结果按照MEC90浓度从高到低,米卡芬净对紫色毛癣菌和断发毛癣菌的MEC90是0.25μg/mL;对犬小孢子菌、疣状毛癣菌的MEC90为0.06μg/mL;对红色毛癣菌、须癣毛癣菌、石膏小孢子菌、絮状表皮癣菌的MEC90均在0.03μg/mL。②卡泊芬净对红色毛癣菌、紫色毛癣菌和断发毛癣菌的MEC90为1μg/mL;对须癣毛癣菌、犬小孢子菌、石膏小孢子菌、絮状表皮癣菌和疣状毛癣菌的MEC90为0.5μg/mL。③根据中位数检验,米卡芬净对几种皮肤癣菌的MEC值均低于卡泊芬净的MEC值,统计学比较有显著性差异(P<0.05)。结论米卡芬净和卡泊芬净对皮肤癣菌有较强的抑菌作用,米卡芬净的MEC值低于卡泊芬净。

Objective To investigate the in vitro antifungal activity of two new echinocandin antifungal drugs, micafungin and caspofungin, against dermatophyte. Methods Minimal effective concentrations （MECs） of micafungin and caspofungin to 82 dermatophyte strains were tested according to CLSI （Clinical and laboratory Standards Institute, formerly NCCLS） M38-A2 broth microdilution methods. Results ①From high to low, the MEC90s of Micafungin for T. violaceum and T. tonsurans were 0. 25 μg/mL,followed by those for M. canis and T. verrucous with the MEC90s of 0.06 μg/mL. The MEC90s for T. rubrum,T, mentagrophytes,M. gypseum and E.floccosum were 0.03 μg/mL. ②The MEC90s of Caspofungin for T. rubrum, T. violaceum and T. tonsurans were 1 μg/ mL, followed by those for T. mentagrophytes ,M. canis ,M. gypseum,E.floccosum and T. verrucous with the MEC90s of 0.5μg/mL. ③ Micafungin exhibited more effective antifungal activity to dermatophytes than caspofungin statistically （ P 〈 0.05 respectively）. Conclusion Micafungin showed stronger antifungal activity than caspofungin with lower MEC.