泰素联合放射对人盲肠未分化腺癌离体细胞系的作用

The combined effects of taxol and radiation on cell proliferation of human cecum undifferentiated adenocarcinoma cell line hce8693 in vitro

目的了解泰素和放射对人盲肠未分化腺癌细胞系（Ｈｃｅ８６９３）的联合作用效应。材料与方法应用ＭＴＴ比色法，细胞核分裂指数，ＤＮＡ琼脂糖电泳及流式细胞仪检测技术。结果泰素对该细胞的抑制呈一定的剂量依赖性；低浓度的泰素（１０μｇ／ｍｌ）可致分裂细胞明显增加；单纯泰素（２０μｇ／ｍｌ）和单纯放射作用后的１８小时均产生细胞凋亡现象，而细胞经泰素联合放射作用后的３～２４小时均出现了ＤＮＡ梯状（Ｌａｄｄｅｒ）条带及凋亡（Ａｐｏｐｔｏｓｉｓ）小峰。结论泰素结合放射有一定协同作用，至少有相加效应。

Purpose To study the adjuvant effects of toxal in different concentrations on cell proliferation of human cecum undifferentiated adenocarcinoma cell line Hce8693 in vitro.Materials and Methods MTT assay,cell nucleus division index,DNA agrose electrophoresis and flowcytometry.Results The results showed that the effects of taxol on cell proliferation were dosedependent.The enhancement of the ratio of mitosis in cells could be induced by taxol with low concentration(10μg/ml).Apoptosis could be induced in cells 18h after treatment with taxol or radiation alone,but DNA“Ladder” and Apoptosis peak in FCM both appeared in cells after treatment with taxol combined with radiation for 3～24 hours.Conclusion Taxol combined with radiation shows the synergistic or additive killing effect in vitro on Hce8693 cell line.