摘要
目的了解泰素和放射对人盲肠未分化腺癌细胞系(Hce8693)的联合作用效应。材料与方法应用MTT比色法,细胞核分裂指数,DNA琼脂糖电泳及流式细胞仪检测技术。结果泰素对该细胞的抑制呈一定的剂量依赖性;低浓度的泰素(10μg/ml)可致分裂细胞明显增加;单纯泰素(20μg/ml)和单纯放射作用后的18小时均产生细胞凋亡现象,而细胞经泰素联合放射作用后的3~24小时均出现了DNA梯状(Ladder)条带及凋亡(Apoptosis)小峰。结论泰素结合放射有一定协同作用,至少有相加效应。
Purpose To study the adjuvant effects of toxal in different concentrations on cell proliferation of human cecum undifferentiated adenocarcinoma cell line Hce8693 in vitro.Materials and Methods MTT assay,cell nucleus division index,DNA agrose electrophoresis and flowcytometry.Results The results showed that the effects of taxol on cell proliferation were dosedependent.The enhancement of the ratio of mitosis in cells could be induced by taxol with low concentration(10μg/ml).Apoptosis could be induced in cells 18h after treatment with taxol or radiation alone,but DNA“Ladder” and Apoptosis peak in FCM both appeared in cells after treatment with taxol combined with radiation for 3~24 hours.Conclusion Taxol combined with radiation shows the synergistic or additive killing effect in vitro on Hce8693 cell line.
出处
《中华放射肿瘤学杂志》
CSCD
北大核心
1998年第1期25-28,共4页
Chinese Journal of Radiation Oncology
关键词
盲肠肿瘤
腺癌
Hce-8693
放射疗法
泰素
Taxol Radiation Cell proliferation Human cecum undifferentiated adenocarcinoma cell line Hce8693