人反义c-myc重组逆转录病毒表达载体pLNC-aM1的构建

Construction of a Human Antisense cmyc Recombinant Retrovirus Expression Vector pLNC-aM1

ｃ－ｍｙｃ第一外显子在ｃ－ｍｙｃ转录调节中起着重要作用。为了解ｃ－ｍｙｃ第一外显子的生物学效应，探讨ｃ－ｍｙｃ的转录调控机制，为ｃ－ｍｙｃ表达增高性疾病的基因治疗提供依据，我们采用分子克隆技术，将１．３ｋｂ的人ｃ－ｍｙｃ第一外显子片段经克隆载体ｐＵＣ１９换得适宜克隆位点，回收目的片段再反向导入逆转录病毒表达载体ｐＬＮＣＸ，构建成人ｃ－ｍｙｃ第一外显子反义载体ｐＬＮＣ－ａＭ１。

xon 1 of cmyc plays a key role in cmyc transcription control. In order to investigate the biology effect of cmyc exon 1, determine the mechanism of cmyc transcription regulation, and then to provide evidents for the gene therapy of cmyc overexpression diseases, we used gene clone technology to construct 1.3kb human cmyc exon 1 fragment into cloning vector pUC19 to gain proper cloning sites, then recovery the target fragment and put into retrovirus expression vector pLNCX in reverse direction to construct human antisense cmyc exon 1 recombinant vector pLNC-aM1.