大鼠胎脑细胞脊髓内移植的实验研究

Experimental Study on Transplantation of Fetal Brain into Spinal Cord of Adult Rats

本文是用孕１３天（Ｅ１３）大鼠胎脑细胞悬液，以慢速注入法移植于同种成年大鼠脊髓白质的侧索及后索、中央管及灰质中。移植后分１天、２周、４周、１２周等４组，用光镜检查在脊髓内不同部位移植的胎脑细胞，存活、生长、分化、成熟以及与宿主脊髓嵌合等情况。结果表明：①移植于成鼠脊髓侧索、后索及中央管等不同部位，９０％以上移植成功，胎脑细胞的存活、生长、分化极为良好，术后４周形成神经毡样结构，且多与宿主灰质嵌合；②灰质内移植组的宿主脊髓灰质损伤严重，神经元多变性、坏死消失；③移植于脊髓不同部位的方法将有助于脊髓前、后以及中央等各种类型损伤的修复，探讨是否有可能建立脊髓内不同移植部位的模型。

n this report, we used the slow injection method to transplant the fetal brain cell suspension of E 14 rats into the funiculus lateralis and the funiculus posterior of the white matter, canalis centralis and the grey matter in the spinal cord of adult rats. After transplantation, the rats were divided into four groups:one day, two weeks, four weeks and twelve weeks after transplantation. By light microscopy, we examined the transplanted fetal brain cells in different parts of the spinal cord, assessed their survival, growth, differentiation, maturation and integration with the host spinal cord. The results were as follows: ① After we slowly injected the fetal brain cell suspension of E 13 rats into the funiculus posterior, funiculus lateralis and canalis centralis of the spinal cord of adult rats, 90 percent of them were successfully transplanted, the fetal brain cells survived, grew and differentiated perfectly well, developed into neuropil structure in four weeks after the operation. Most of them integrated with the grey matter of the host, constructing relaying morphological basis. ②While the transplantation was made in the grey matter, the grey matter of the host spinal cord was severely impaired and the most of the neurons degenerated, and diminished. ③The transplantation of the fetal brain cell suspension into the different portions of the spinal cord of adult rats, benefit the recovery of various injuries involved in the anterior, posterior and centrical portions of the spinal cord.