病毒性心肌炎不同时点血管内皮细胞生长因子mRNA的表达

Expression of vascular endothelial growth factor in mice with Coxsackie virus B3-induced viral myocarditis

目的探讨血管内皮细胞生长因子(VEGF)在BALB/c小鼠感染柯萨奇病毒B3(CVB3)所致病毒性心肌炎(VM)病程中的作用。方法取BALB/c小鼠70只,随机分为VM组60只,腹腔感染CVB3,分别于实验第3、7、10、14、18、21天取心肌组织;正常组10只,于实验第21天取心肌组织。用逆转录聚合酶链反应(RT-PCR)技术检测其心肌组织VEGFmRNA的表达。结果病毒性心肌炎小鼠心肌VEGFmRNA表达较正常组高,且在感染病毒后表达渐增高,14d达最高峰,其后表达降低,但仍维持一较高水平,与正常组比较仍有统计学意义(P<0.01)。VEGFmRNA表达与心肌病理损害程度密切相关。结论VEGF可能参与BALB/c小鼠病毒性心肌炎的病理生理过程。

Objectives To investigate the changes of vascular endothelial growth factor （VEGF） in BALB/c mice with viral myocarditis （VM）, and to explore the role of VEGF in VM. Methods Seventy BALB/c mice were randomly divided into 2 groups： VM group （n = 60） and control group （n =10）. Mice in VM group were inoculated intraperitoneally with Coxsackie virus B3 （CVB3） while control mice with 0.1 ml of Eagle＇s minimal essential medium （EMEM）. Myocardial musculature of VM mice were collected on the day of 3, 7, 10, 14, 18 and 21 after inoculation. Myocardial musculature of control mice were collected on the day of 21 after inoculation. Histological cross sections of heart were stained with hematoxylin-eosin and myocardial histopathologic scores were counted under optical microscope. The expression of VEGF mRNA was detected by reverse transcription polymerase chain reaction. Results The expression of VEGF mRNA in mice with VM was dynamic： gradually increased after mice being infected with virus, and reached its top on day 14, then decreased, but still keep at a high level. The expression had significant difference between VM group and control group （P 〈 0.01 ）. The myocardiocytes VEGF mRNA was closely correlated with the myocardial histopathologic score. Conclusions The changes of myocardial VEGF mRNA were associated with myocardial pathologic lesion of VM, that may contribute to pathophysilogical procedure of VM. The present findings may indicate that the augmented production of VEGF play a role in ameliorating actions on the injured heart in CVB3 myocarditis mice.