高效液相色谱法测定甘薯叶中绿原酸含量

Determination of chlorogenic acid constituent in sweet potato leaves by HPLC

为准确测定甘薯叶中绿原酸含量,通过摸索流动相、洗脱方式、保留时间和速度等条件,建立了测定甘薯叶中绿原酸含量的高效液相色谱方法.采用色谱柱Lichrospher5-C18柱(150 mm×4.6 mm,5μm),以A(水∶乙腈∶冰醋酸=980∶20∶5),B(乙腈∶冰醋酸=1 000∶5)为流动相,线性梯度洗脱,流速0.3 mL/min,检测波长324 nm.结果表明,绿原酸在5～100μg/mL时,绿原酸质量浓度与峰面积呈良好的线性关系,回归方程为y=195.47x+144.29(r=0.999 1),平均回收率可达93.1%.

To determine accurately the content of chlorogenic acid in different types of sweet potato leaves, HPLC method was established by groping the mobile phase, elution mode, retention time and speed conditions. Separation was carried out on a Lichrospher5 - C18 column（150 mm×4.6 mm, 5 μm） andA（water ： acetonitrile ： acetic acid=980 ： 20 ： 5） and B（acetonitrile ： acetic acid=l 000 ： 5） as the mobile phase were used. The flowing rate was 0.3 mL/min and detection was affected at 324 nm. The experimental results showed that the assay displayed good linearity over the concentration range of 5 - 100 μg/mL and the average recovery was 93.1%.