越南进境人参果炭疽病病原菌的分离鉴定

Isolation and identification of Colletotrichum gloeosporioides on pepino fruits from Vietnam.

2008年4月从广州出入境检验检疫局送检的越南进境人参果样品(报检号:P02297)中发现果实表面密布近圆形褐色病斑,病斑直径约为3mm,中央黄褐色,边缘深红褐色,多个病斑可愈合成片。室温下放置1周,病果即完全腐烂,全果果皮及果肉均转成褐色。采用组织分离培养法获得分离物,经对分离物培养性状与形态特征观察、致病性测定,并辅助于分子生物学检测,将病害鉴定为人参果炭疽病,病原菌为胶孢炭疽菌(Colletotrichum gloeosporioides)。B last分析表明从病原菌基因组中扩增到的1.8 kbp 18S rDNA片段与胶孢炭疽菌18S rRNA基因同源率为99%。迄今为止人参果炭疽病在国内外均未见报道,这是我国首次从进境人参果中截获并检验鉴定该病害。

In April 2008, some rot fruits on pepino （Solanum muicatum Aiton. ） from Vietnam were intercept by Guangzhou Exit- Entry Inspection and Quarantine Bureau. Some symptoms were observed on the fruits. On the surface of the fruits were some round brown spots with a diameter of approximately 3 ram. In the middle of the spot was yellow brown, and on the edge was dark red brown. Within one week, all fruits have rotted away and both skin and flesh of fruits turned brown. The causal fungus was isolated from the skin of decayed fruit by peeled from anthracnose on pepino fruits caused by C. gloeosporioides has not been recorded in China and overseas. The interception and identification of Anthracnose （ C. gloeosporioides ） on pepino fruits is the first time in China.