小麦矮秆种质系山农495矮秆基因的分子标记定位

Developing Molecular Markers for the Rht Gene in Dwarfing Germplasm Line Shannong 495

为给新矮秆种质系山农495的利用提供依据,利用田间观察和室内赤霉酸鉴定与分子标记分析相结合的方法,进行矮秆性状的遗传分析和矮秆基因分子标记定位。结果表明,山农495的矮秆性状受位于4BS染色体上的1对隐性主效基因控制,且为赤霉酸不敏感型。在供试的1 589个SSR引物中,有2个基因组SSR引物(Gwm113196、Wmc 511191)和1个EST-SSR引物(Dupw23210)在优选小群体中能扩增出多态性谱带。利用BC1回交群体和F2群体进行连锁标记分析,Gwm113196、Wmc 511191和Dupw23210与山农495矮秆基因的连锁距离分别为3.9、5.2、21.6 c M和4.1、5.0、19.8 c M。

In this study, the genetic characteristic of Rht gene in Shannong 495 was studied by field investigation, GA reaction identification and molecular marker. The results showed that the dwarfing gene in Shannong 495 may be a recessive gene on chromosome 4BS; this dwarfing gene was insensitive to GA; 1 589 genomic SSR and EST-SSR markers were screened for polymorphism in the Preferred Small Group （PSG） and two genomic SSR markers （Gwrn 113198 and Wmc 511 191 ） and one EST-SSR marker （Dupw 23210） were identified polymorphic. These polymorphic markers were used to screen the Rht gene in BC1 and F2 segregation population, and all the three polymorphic markers were linked to this dwarf gene. The genetic distance between this Rht gene and microsatellite markers of Gwm 113196, Wrnc 511 191 and Dupw 2321o was 3.9,5.2 and 21.6 cM using BC1 population, respectively, and 4. 1,5.0 and 19.8 cM using F2 population, respectively.