摘要
采用组织培养和常规石蜡切片技术,研究了在组培条件下条叶龙胆试管苗嫩茎扦插繁殖过程中不定根的发生与发育,结果表明,条叶龙胆茎上的不定根起源于形成层,在生根培养基上不产生愈伤组织,不经过愈伤脱分化阶段,属直接器官再生.
The tissue culture and wax section were used in this study. The origin and development of adventitious roots formed in fresh stern of Gentiana manshurica in tissue culture were studied. The results show that the adventitious roots are developed in the cambium of the stem. The fresh stem dose not form callus in the culture medium inducting roots and the dedifferentiation was not observed. The development of adventitious roots is direct organogenesis.
出处
《哈尔滨师范大学自然科学学报》
CAS
2009年第1期71-73,85,共4页
Natural Science Journal of Harbin Normal University
基金
黑龙江省教育厅科学技术研究项目(11511123)
哈尔滨市培养学科后备带头人基金(2005AFXXJ032)
哈师大博士科研启动基金项目
关键词
条叶龙胆
组织培养
扦插
不定根
根原基
Gentiana manshurica
Tissue culture
Cutting
Adventitious root
Root primordium
