基质金属蛋白酶MMP-9及其组织抑制剂TIMP-1mRNA在卵巢上皮性肿瘤中的表达及其临床意义(英文)

Expression of MMP-9 and TIMP-1 in epithelial ovarian tumors and their clinical significance

目的探讨基质金属蛋白酶(MMP-9)及其组织抑制物(TIMP-1)mRNA与卵巢上皮性癌的发生、发展的关系。方法采用RT-PCR技术检测69例卵巢上皮性肿瘤(38例恶性、15例交界性、16例良性)及11例正常卵巢组织中MMP-9及TIMP-1mRNA的表达,并分析其与各临床病理特征的关系。结果MMP-9mRNA在卵巢上皮性癌及交界性肿瘤组织中的阳性表达率(分别为76%、44%)及表达水平[分别为(154.14±4.42)、(96.87±3.04)]均显著高于良性肿瘤及正常卵巢组织[分别为13%、9%和(8.26±2.67)、(2.34±1.02);P<0.05];且MMP-9mRNA在晚期卵巢上皮性癌组织中的阳性表达率和表达水平明显高于早期癌组织(P<0.05)。TIMP-1mRNA在卵巢上皮性癌及交界性肿瘤组织中的阳性表达率(分别为68%、38%)及表达水平[分别为(126.35±3.81)、(90.62±5.54)]均显著高于良性肿瘤及正常卵巢组织[分别为13%、9%和(8.32±2.02)、(2.63±1.35);P<0.05];TIMP-1mRNA在卵巢上皮性癌组织中的阳性表达率和表达水平与各临床病理特征均无相关性(P>0.05)。结论MMP-9mRNA的高表达及MMP-9/TIMP-1平衡的调节失衡导致卵巢上皮性肿瘤的发生、侵袭和转移;MMP-9mRNA的高表达对卵巢上皮性癌有预后意义。

[Objective] To investigate the correlations of matrix metalloproteinase-9 （MMP-9） and tissue inhibitor of matrix metalloproteinase-1 （TIMP-1） mRNA with tumorigenesis and progression of epithelial ovarian carcinoma （EOC）. [Methods] Reverse transcriptase polymerase chain reaction （RT-PCR） was performed to evaluate mRNA expression of MMP- 9 and TIMP- 1 in 69 cases of epithelial ovarian neoplasm （38 malignant, 15 borderline and 16 benign） as well as 11 cases of normal ovarian tissues, and explore their correlations with clinieopathologic characteristics. [Results] The positive rates （76%, 44%, respectively） and semiquantitative levels [（154.14±4.42）, （96.87±3.04）, respectively] of MMP- 9 mRNA expression were significantly higher in EOC and borderline tumors than those in benign tumors and normal ovarian tissues [13%, 9%, respectively; and （8.26±2.67）, （2.34±1.02）, respectively, P 〈 0.05]. The expression level of MMP- 9 mRNA in advanced stage EOC was significantly higher than that in early stage EOC （P 〈0.05）. The positive rate （68%, 38%, respectively） and semi-quantitative level [（126.35±3.81）, （90.62± 5.54）, respectively] of TIMP-1 mRNA were significantly higher in EOC and borderline tumors than those in benign tumors and normal ovarian tissues [13%, 9% and （8.32±2.02）, （2.63±1.35）, respectively; P 〈0.05]. No correlations were found in positive rate and semi-quantitative level of TIMP-1 mRNA expression with clinicopathologie characteristics in EOC. [Conclusions] The overexpression of MMP-9 mRNA and the imbalance between MMP- 9 and TIMP- 1 may lead to tumorigenesis, invasion and metastasis in epithelial ovarian tumor. Overexpression of MMP- 9 mRNA may have prognostic significance in EOC.