p,p’-DDE对离体培养的大鼠睾丸支持细胞内钙离子的影响

Effects of p,p’-DDE on Intracellular Calcium in Sertoli Cells of Rats in vitro

目的研究p,p’-DDE对离体培养大鼠睾丸支持细胞(Sertoli cell)细胞内钙离子的影响。方法从大鼠睾丸组织中分离支持细胞并进行离体原代培养,设空白对照组、溶剂对照组和低、中、高剂量p,p’-DDE染毒组(10、30、50μmol/L),然后以Fura-2/AM为钙探针,用细胞内双波长钙荧光系统通过测定荧光值计算细胞内钙离子浓度的变化。结果高剂量组支持细胞中Ca2+浓度高于溶剂对照组,差异有统计学意义(P<0.05)。结论p,p’-DDE可干扰支持细胞内钙离子稳态,造成钙的超负载。钙离子稳态失衡可能诱导支持细胞凋亡,最终可能导致生殖功能障碍。

Objective To study the effects of p,p＇ -DDE on intracellular calcium in the Sertoli cells of rats in vitro. Methods The primary culture of the Sertoli cells separated from the testicle tissues of rats was conducted, and the cells were treated with p,p ＇-DDE at the doses of 10, 30 and 50 umo]/L. Fura-2/AM was used as the probe to examine the changes of intracellular calcium by two-wavelength calcium fluorescence system. Results Compared with the control, in 50 umo]/L group of p,p＇-DDE, the intracellular calcium level significantly increased. Conclusion p,p＇-DDE may disrupt intracellular calcium homeostasis which will induce calcium overload in Sertoli cells in the testicle tissues of SD rats.