纳米铜对大鼠肝脏和肾脏的氧化损伤作用

Oxidative Damage in Livers and Kidneys Induced by Nano-copper Particles in Rats

目的比较纳米铜与微米铜对大鼠肝脏和肾脏的氧化损伤,探讨氧化损伤在纳米铜致大鼠肝毒性和肾毒性中的作用。方法SPF级雄性Wistar大鼠30只,随机分为溶剂对照组(1%羟丙甲基纤维素),微米铜组(200 mg/kg),纳米铜3个不同剂量组(50、100和200 mg/kg),每组6只,10 ml/kg经口灌胃染毒,每日一次,连续5 d。染毒结束后,留取大鼠肝脏和肾脏用硫代巴比妥酸法(TBA)测定丙二醛(MDA)含量,二硫代双硝基苯甲酸法(DTNB)测定总巯基(TSH)和非蛋白巯基(NPSH)含量。结果纳米铜染毒组大鼠肝脏和肾脏组织中NPSH的含量随染毒剂量增加先增后降,而MDA含量呈剂量依赖性增加,尤其是在纳米铜高剂量组(200 mg/kg),与对照组比较,差异有统计学意义(P<0.05,P<0.01),而在微米铜组却未发现相应改变。结论在相同剂量和暴露时间条件下,纳米铜对大鼠的毒性明显强于微米铜,纳米铜导致大鼠肝脏和肾脏损伤的机制可能与肝、肾组织中NPSH的耗竭及脂质过氧化有关。

Objective To study the oxidative damage in the livers and kidneys of rats induced by nano-copper particles. Methods Thirty male Wistar rats were randomly assigned into five groups, 6 in each, the rats were given nano-copper at the doses of 50, 100 and 200 mg/kg and micro-copper at 200 mg/kg, the control was given the vehicle, 1% hydroxypropylmthylcellulose, respectively, by gavage, once a day, for consecutive 5 days. The rats were sacrificed on day 6, the content of total sulfhydryl （TSH）, non-proteins ulfhydryl （NPSH） and malondialdehyde （MDA） in the livers and kidneys were measured respectively. Results The content of NPSH in the livers and kidneys increased in low dose nano-copper group, but decreased in high dose nano-copper group. The MDA level significantly increased in nano-coppergroups in dose-dependent manner, especially in high close group （P〈0.05 or P〈0.01）. But in micro-copper treated group, no significant change was seen in the content of TSH, NPSH and MDA. Conclusion The hepatotoxicity and nephrotoxicity induced by high dose nano-copper （200 mg/kg） in rats should be related to the depletion of NPSH and lipid superoxidation.