摘要
目的建立祛痘除螨类化妆品中7种常见抗生素(盐酸米诺环素、盐酸土霉素、盐酸四环素、盐酸金霉素、盐酸多西环素、氯霉素及甲硝唑)同时测定的超高效液相色谱法。方法样品中待测物采用甲醇与1%甲酸水溶液等体积混合液超声提取,色谱柱采用Waters UPLC BEH C18流动相A(甲醇:乙腈体积比为1:2)与B(1%甲酸水溶液)梯度洗脱,流量为0.2ml/min,检测波长为268nm。结果该方法各组分检出限为0.3~0.5ng,线性范围为2~100ng(1-50mg/L),RSD≤4.8%,加标回收率为82.5%~105.0%。结论该方法快速,灵敏,能够满足检测祛痘除螨类化妆品中7种常见抗生素的需要。
Objective To establish an ultra performance liquid chromatography method for the determination of 7 kinds of antibiotics (minocycline hydrochloride, ox tetracycline, tetracycline hydrochloride, chlortetracycline hydrochloride, doxycycline hydrochloride, chloramphenicol, metronidazole) in anti-acne cosmetics. Methods Seven kinds of antibiotics were separated on a Waters UPLC BEH C18 column by gradient elution using A (methanol/acetonitrile = 1/2) and B(1% formic acid aqueous solution) as the mobile phase at a flow rate of 0.2 ml/min, with the detection wavelength of 268 nm. Results The detection limits were 0.3-0.5 ng, the calibration curves were linear in the range of 2-100 ng (1-50 mg/L), the precision was not more than 4.8% and the rate of recovery were 82.5%-105.6%. Conclusion The method established in the present paper is rapid, sensitive and suitable for the determination of 7 kinds of antibiotics in anti-acne cosmetics.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2009年第5期453-454,共2页
Journal of Environment and Health
基金
江苏省卫生厅预防医学基金资助项目(Y200709)
关键词
化妆品
色谱法
液相
超高效液相色谱法
抗生素
Cosmetics
Chromatography,gas
Ultra performance liquid chromatography
Antibiotics