化妆品中7种常见抗生素的超高效液相色谱测定法

Determination of Seven Antibiotics in Cosmetics by Ultra Performance Liquid Chromatography

目的建立祛痘除螨类化妆品中7种常见抗生素（盐酸米诺环素、盐酸土霉素、盐酸四环素、盐酸金霉素、盐酸多西环素、氯霉素及甲硝唑）同时测定的超高效液相色谱法。方法样品中待测物采用甲醇与1％甲酸水溶液等体积混合液超声提取，色谱柱采用Waters UPLC BEH C18流动相A（甲醇：乙腈体积比为1：2）与B（1％甲酸水溶液）梯度洗脱，流量为0．2ml／min，检测波长为268nm。结果该方法各组分检出限为0．3～0．5ng，线性范围为2～100ng（1-50mg／L），RSD≤4．8％，加标回收率为82．5％～105．0％。结论该方法快速，灵敏，能够满足检测祛痘除螨类化妆品中7种常见抗生素的需要。

Objective To establish an ultra performance liquid chromatography method for the determination of 7 kinds of antibiotics （minocycline hydrochloride, ox tetracycline, tetracycline hydrochloride, chlortetracycline hydrochloride, doxycycline hydrochloride, chloramphenicol, metronidazole） in anti-acne cosmetics. Methods Seven kinds of antibiotics were separated on a Waters UPLC BEH C18 column by gradient elution using A （methanol/acetonitrile = 1/2） and B（1% formic acid aqueous solution） as the mobile phase at a flow rate of 0.2 ml/min, with the detection wavelength of 268 nm. Results The detection limits were 0.3-0.5 ng, the calibration curves were linear in the range of 2-100 ng （1-50 mg/L）, the precision was not more than 4.8% and the rate of recovery were 82.5%-105.6%. Conclusion The method established in the present paper is rapid, sensitive and suitable for the determination of 7 kinds of antibiotics in anti-acne cosmetics.