摘要
目的对杨梅素诱导人肝癌HepG2细胞凋亡机制进行研究。方法采用MTT法测定细胞死亡率;采用倒置显微镜观察细胞形态学变化;用琼脂糖电泳观察DNA片段化。用Western印迹法分析相关蛋白的变化。结果杨梅素明显抑制HepG2细胞的增殖,诱导HepG2细胞调亡。Caspase-3,caspase-9抑制剂可明显抑制100μg/ml杨梅素诱导的凋亡。Western印迹结果显示100μg/ml杨梅素作用于细胞后,细胞色素c水平明显升高。结论杨梅素(100μg/ml)通过激活线粒体途径诱导HepG2细胞凋亡。
Objective To study the apoptosis inducing effect of myricetin on human hepatocellular carcinoma HepG2 cell. Methods Cell viability was measured by MTT method. Morphological changes were observed by phase contrast microscopy and Hoechst 33258 staining. DNA fragmentation was assayed by agarose gel electrophoresis. Protein level was detected by Western blot analysis. Results Morphological changes and DNA fragmentation showed that myricetin induced HepG2 cell apoptosis. The apoptosis of HepG2 cells was partially reversed by caspase-3 and-9 inhibitors. Treatment of HepG2 cells with myricetin for 24 h induced up-regulation of cytochrome c. Conclusion Myricetin induced HepG2cell death via activation of mitochondria pathway.
出处
《中国现代药物应用》
2009年第10期1-3,共3页
Chinese Journal of Modern Drug Application
关键词
杨梅素
肝癌
凋亡
Myricetin
HepG2 cell
Apoptosis
