2型糖尿病患者大血管并发症与DNA氧化损伤的关系研究

Study on the relationship between DNA oxidative damage and macrovascular complications in patients with type 2 diabetes

目的探讨2型糖尿病患者大血管并发症与DNA氧化损伤的关系。方法选择78例2型糖尿病患者,32例合并有大血管并发症(A组),46例无并发症(B组),检测患者的血三酰甘油(TG)、胆固醇(TC)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)、糖化血红蛋白(HbA1c)及8-羟基脱氧鸟嘌呤(8-OHdG)水平。8-OHdG检测采用酶联免疫吸附法。结果A组血8-OHdG水平(2.93±1.37)μg/L,B组(2.67±1.30)μg/L,2组比较无显著性差异;A组TG水平(1.99±0.98)mmol/L,明显高于B组的(1.54±0.89)mmol/L(P<0.05);A组病程(13.41±7.76)a,明显高于B组的(7.22±5.77)a(P<0.05)。血8-OHdG水平与病程、TG呈正相关关系,而与TC、LDL-C、HDL-C及HbA1c水平无明显相关关系。结论2型糖尿病患者大血管并发症的发生可能与高血糖所致氧化应激关系不大,而与血脂关系更密切;脂代谢异常是糖尿病氧化应激的重要影响因素。

Objective It is to explore the relationship between DNA oxidative damage and macrovascular complications in patients with type 2 diabetes. Methods 78 cases of patients with type 2 diabetes were chosen and divided into group A including 32 cases with macrovascular complications and group B including 46 cases without the complications. Serum levels of TG, TC, LDL- C, HDL- C, hemoglobin Ale （HbAlc） and 8 - hydroxy - deoxyguanosine （8 - OHdG） in both groups were tested. 8- OHdG was detected by enzyme-linked immunosorbent assay （ELISA）. Results There was no significant difference in 8 - OHdG level between group A in which the level was （2.93 ± 1.37）μg/L.and group B in which was （2.67 ± 1.30） μg/L. The level of TG in group A was （1.99 ± 0.98） mmol/L, which was higher than that in group B which was （ 1.54 ± 0.89）mmol/L（ P 〈 0.05 ）. The disease course in group A was（ 13.41 ± 7.76）a and was longer than that in group B which was （7.22 ± 5.77） a （ P 〈 0.05 ）. 8 - OHdG level was positively correlated with the disease course and TG （ P 〈 0.05）, and had no obvious correlation with the levels of TC, LDL - C, HDL - C and HbA1c. Conclusion The role of oxidative stress in type 2 diabetes with maerovascular complications may not be the most important, the lipid level of the patients may be more closely related with it. Abnormal lipid metabolism is the important influencing factor of oxidative stress in diabetes.