^(125)I-脱氧尿嘧啶核苷瘤内注射治疗人膀胱癌的动物实验研究

Animal experimental study of intratumoral injection using ^(125)I-UdR on human bladder cancer

目的研究125I-脱氧尿嘧啶核苷(125I-UdR)在荷人膀胱癌裸鼠移植瘤模型中的应用效果和安全性,为125I-UdR临床应用提供实验依据。方法荷人膀胱癌裸鼠移植瘤瘤内注射相对合适剂量(123.33 MBq/kg)的125I-UdR,通过肿瘤生长抑制率、肿瘤病理及电镜改变以及γ计数仪测量各脏器放射性活度来分析其疗效和安全性。结果荷人膀胱癌裸鼠移植瘤二次瘤内注射相对合适剂量的125I-UdR,肿瘤体积缩小明显,病理示坏死彻底(核消失),电镜见核溶解、消失。外周血常规及肝肾功能无明显变化,正常组织无明显病理改变。结论荷人膀胱癌裸鼠二次瘤内注射125I-UdR(123.33 MBq/kg)是安全有效的。

Objective It is to investigate the pharmic safety and curative effect of ^125I- UdR in athymic mouse model bearing bladder cancer for the preparation of clinic application of ^125I - UdR. Methods A proper dosage （ 123.33 MBq/kg） of ^125I - UdR was injected into the transplanted tumor of athymic mice to investigate the pharmic safety and curative effect of ^125I- UdR by the tumor growth inhibition ratio, the pathological changes, electronic microscope changes of tumor and radioaction activity of various organs with 7 scintillation counter. Results The tumor bulk had contracted obviously after injection of ^125I - UdR （123.33 MBq/kg） for twice time. Pathology indicated thorough necrosis. The dissolve and abolition of cell nucleus could be found under electron microscope. The changes of hemogram and biochemistry indexes were not observed, no significant pathological changes were found in normal organs. Conclusion It is safe and effective to inject ^125I - UdR （123.33 MBq/kg） into the transplanting tumor of tumor- bearing mouse with bladder cancer for twice time.