蛋白质组学在研究植物响应逆境机理上的应用

Application of proteomics technology on researches of adaptation mechanisms of plants to adverse stresses

逆境条件下植物可以通过改变其基因表达和相关代谢活动来适应,探讨植物基因和蛋白表达谱的变化就成为植物逆境响应机制研究中的重要内容,蛋白质表达谱反映了植物细胞和组织的实际状态,是植物基因表达和最终代谢的关键环节。随着蛋白质分离技术、质谱鉴定技术和植物生物信息学的迅速发展,蛋白质组学在植物响应逆境方面的研究中的应用已经比较成功,加深了人们对植物响应逆境机制的认识,并为人们提供了新的线索和思维。本文主要对蛋白质组学在植物响应非生物逆境(干旱、盐胁迫、低温胁迫、高温胁迫等)和生物逆境(病虫害)的机制研究的应用上进行了综述。

Expression profiling is an important tool to investigate how plant responds to environmental changes. Plants have the ability to alter their gene expression patterns dramatically in response to environmental changes. The proteome profile reflects the actual state of the cell or the tissue of plant and is an essential bridge between the gene transcription and the actual metabolism. In recent years,due to the rapid developments of techniques in protein separation and protein identification technique based on Mass Spectrometry and Bio-irformaics, the proteomics technique has become an important technical approach for plant researchers to assess the changes in protein types and their expression levels under different stresses,and many new insights were provided by using this technique. Present paper reviewed the research progresses of plant proteomics on plant responses to abiotic or biotic stresses, including high temperature, low temperature, salt, drought, pathogens and insects