一个水稻OsWNK基因的分离及表达分析

Isolation and expression analysis of an OsWNK gene in rice

在褐飞虱取食后的水稻cDNA差减文库中筛选到与拟南芥AtWNK1激酶基因高度同源的EST(GenBank登录号:BU572310),以该EST为探针,从褐飞虱取食后的水稻cDNA文库中分离到OsWNK基因的全长cDNA,该基因编码一个含677个氨基酸残基的蛋白激酶,与以前克隆出的一种拟南芥蛋白激酶基因(GenBank登录号:DQ837532)只有3个氨基酸残基的差异。Northern杂交结果显示,在褐飞虱取食后,该基因的表达上升。表明该激酶基因参与褐飞虱取食的应答反应,可能与水稻抗褐飞虱有关.

BPHiw008, an EST （expressed sequence tag） which is highly homologous with Arabidopsis WNK 1 （with no lysine kinase）,has been screeded from the rice cDNA SSH library. Using this EST as probe to screen the cDNA library induced by brown planthopper sucking, a full length of cDNA with 2.4 Kb in size was isolated and named as OsWNK. OsWNK is deduced to encode a 677-amino-acid protein OsWNK through BLAST. Only 3 amino-acid residues of OsWNK are different from those of AtWNK1. Result of RNA gel blot analysis showed that OsWNK was up-regulated in brown planthopper-sucked rice, indicating that OsWNK may play a role in resistance to brown planthopper of rice.