实时荧光定量PCR(TaqMan)法测定外源基因的拷贝数

Estimating copy number of transgenic gene by real-time fluorescent quantitative PCR(TaqMan)

实时荧光定量PCR是近年新兴的一项技术,因其快速、方便、便宜,需要DNA样品量少,无需放射性检测等优点被广泛应用于基因的定量分析。该文就实时荧光定量PCR(TaqMan)技术的发展、基本原理及测定外源基因拷贝数的技术流程做一介绍。

Real-time fluorescent quantitative PCR is a new technique which has been developed to be used in quantitative analysis of genes as well with the advantages of simpleness, quickness, short needs of target fragment DNA and none of hazardous radioisotopes in recent years. The development, the principle of real-time fluorescent quantitative PCR（TaqMan） and the techinical flow of estimating copy number of transgenic gene are introuduced in this paper.