CD55、CD59在诊断再生障碍性贫血-阵发性睡眠性血红蛋白尿综合征中的应用

Application of CD55 and CD59 expression in diagnosis of aplastic anemia-paroxysmal nocturnal hemoglobinuria syndrome

目的研究CD55、CD59在再生障碍性贫血-阵发性睡眠性血红蛋白尿(AA-PNH)综合征中的诊断价值。方法用流式细胞仪对19例AA-PNH综合征进行CD55及CD59的检测,同时做酸化血清溶血试验(Ham试验),比较二者的阳性检测率。结果红细胞CD55异常者17例(89.4%),粒细胞CD55异常者19例(100%);红细胞CD59异常者18例(94.7%),粒细胞CD59异常者19例(100%)。Ham试验异常率为26.3%。红细胞和粒细胞CD55、CD59的异常率明显高于Ham试验(P<0.05)。结论CD55、CD59有助于提高AA-PNH综合征的诊断。

Objective To explore the clinical signifinance of CD55 and CD59 expression in diagnosis aplastic anemia-paroxysmal nocturnal hemoglobinuria （AA-PNH） syndrome. Methods The expression of CD55 and CD59 were measured by flow cytometry in 19 cases with AA-PNH syndrome, at the same time the Ham test was done, and the positive detection rates were compared between two methods. Results The expressions of CD55 in red blood cell and white blood cell were found abnormal in 17 cases（89.4% ） and 19 cases （100%）, while the expressions of CD59 in red blood cell and white blood cell were found abnormal in 18 cases（94.7% ） and 19 cases（ 100% ）. The abnormal rate of Hams test was 26.3%. The abmormal rates of CD55 and CD59 were higher than those of Hams test （P 〈 0.05 ）. Conclusion The expression of CD55 and CD59 can improve the diagnosis of AA-PNH syndrome.