超高效液相色谱法测定龙胆药材中的獐芽菜苦苷和龙胆苦苷

Determination of Swertiamarin and Gentiopicroside in Root of Gentiana scabra Bge.by UPLC

目的建立超高效液相色谱（UPLC）法测定龙胆药材中活性成分的方法。方法采用L9（34）正交表安排实验,确定最佳提取条件。色谱柱：Shimadzu C18（3.0 mm×75 mm,1.7μm）;流动相：乙腈-0.1%磷酸（V∶V=5.5∶94.5）,流速0.80 ml/min;检测波长235,270 nm;柱温为40℃。结果獐芽菜苦苷、龙胆苦苷分别在0.154 8～1.858 0 mg/ml,0.322～5.152mg/ml范围内呈良好的线性关系,平均回收率分别为102.09%,RSD=1.68%（n=6）;103.80%,RSD=1.11%（n=6）。结论采用UPLC法控制龙胆质量,操作简单,结果准确,方法可行。

Objective To establish a method for the determination of swertiamarin and gentiopicroside in root of Gentiana scabra Bge. by UPLC. Methods By L9 （ 34 ） orthogonal design, an optimal condition was confirmed. The separation was performed on Shimadzu C18（3.0 mm ×75 mm,1.7μm） column, the mobile phase was Acetonitrile -0. 1% Phosphorylation（V： V = 5.5： 94.5 ）. The flow rate was 0.8 ml/min, the detection ＇wavelength was 235nm, 270nm, and the column temperature was 40℃. Resuits This method had good linearity in the range of 0. 322 -5. 152mg/ml,and the average recovery was102.09 % with RSD of 1.68% for swertiamarin. It had good linearity in the range of 0. 1548 - 1. 858 mg/ml , and the average recovery was 103.80% with RSD of 1.11% for gentiopicroside. Conclusion This method is simple , accurate , and suitable for the quality control of root of Gentiana scabra Bge. by UPLC.