Ⅰ型与Ⅱ型子宫内膜癌细胞抗氧化能力的比较

Antioxidant ability of type I and type II endometrial cancer cells

目的比较Ⅰ型和Ⅱ型子宫内膜癌细胞对氧化应激的抵抗并探讨其机制。方法①H2O2处理Ⅰ型和Ⅱ型子宫内膜癌细胞,噻唑蓝(MTT)法检测两型细胞的增殖水平;②120μmol H2O2和800μmol H2O2分别处理Ⅰ型和Ⅱ型子宫内膜癌细胞,流式细胞仪检测细胞内活性氧(ROS)水平,酶标仪检测细胞内还原型谷胱甘肽(GSH)和氧化型谷胱甘肽(GSSG)水平,分光光度计检测细胞内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)及谷胱甘肽过氧化物酶(GPx)活性。结果在不进行处理的情况下,Ⅰ型子宫内膜癌细胞较Ⅱ型子宫内膜癌细胞ROS水平及CAT活性较低,GSH水平、GSH/GSSG比值及SOD和GPx活性较高(P<0.01)。在氧化应激条件下,Ⅰ型子宫内膜癌细胞GSH水平及GSH/GSSG比值下降,SOD和CAT活性降低(P<0.01);Ⅱ型子宫内膜癌细胞GSH、GSSG水平明显升高,GSH/GSSG比值略有升高,SOD、CAT和GPx活性升高(P<0.01)。结论Ⅱ型子宫内膜癌细胞与Ⅰ型子宫内膜癌细胞相比较,其本身ROS水平较高,细胞内抗氧化系统及抗氧化酶系统对氧化刺激可进行有效应答,细胞不易受到氧化损伤。

Objective To compare the ability of type I and type Ⅱ endometrium cancer cells for resisting oxidative stress and investigate its related mechanism. Method ① Type I and type It endometrium cancer cells were challenged by H2O2, and cell proliferation was determined by the MTT assay. ② Type I or type Ⅱ endometrium cancer ceils were treated with 120 tmaol H2O2 or 800 wnol H2O2, and cellular ROS, GSH, and GSSG levels and SOD, CAT and GPx activities were determined. Results Without H2O2, type I endometrium cancer cells, compared with the type Ⅱ endometrium cancer cells, had a lower ROS level and CAT activity and a higher GSH level, GSH/GSSG ratio, and SOD and GPx activities. Under oxidative stress, the ROS lev- el increased in both type I and type Ⅱ endometrium cancer cells. GSH level, GSH/GSSG ratio, and SOD and CAT activities were decreased in type I endometrium cancer cells while GSH and GSSG levels, GSH/GSSG ratio, and SOD, CAT and GPx activities increased in type Ⅱ endometrium cancer cells. Conclusion Oxidative stress is higher in type Ⅱ endometrium cancer cells than in type I endometrium cancer cells. The oxidation-reduction system and anti-oxidase system in type Ⅱ endometrium cancer cells can effectively respond to oxidative stress, and cells can effectively resist oxidative stress.