无细胞短棒状杆菌制剂联合瘤内缓释化疗对鼠黑色素瘤的抑制效应

Non-cell corynebacterium parvum product combined with slow intra-tumor release of drugs inhibits B16 melanoma in mice

目的探讨无细胞短棒状杆菌制剂(NCPP)联合瘤内缓释化疗对小鼠黑色素瘤的影响。方法给小鼠皮下接种B16肿瘤细胞后建立荷瘤小鼠,随机分成4组,①生理盐水(NS)对照组:瘤内和腹腔注射NS;②缓释治疗组:瘤内注射含Ara-C缓释液;③NCPP治疗组:腹腔注射NCPP;④联合治疗组:腹腔注射NCPP+瘤内注射含Ara-C缓释液。7 d后重复治疗1次,期间观察各组小鼠肿瘤的生长情况;治疗2周处死小鼠,称瘤重量,光镜下观察瘤组织病理变化,采用流式细胞术检测脾内CD4+T细胞、CD8+T细胞数量变化。结果联合治疗能够明显抑制肿瘤的生长,疗效明显优于其他3组(P<0.05);联合治疗组瘤内出现大片坏死区域(P<0.05),联合治疗组的CD8+T细胞数量明显增多(P<0.01)。结论将NCPP和瘤内缓释化疗技术联合应用能有效地抑制肿瘤的生长。

Objective To explore the effects of non-cell corynebacterium parvum product（NCPP） combined with slow intra-tumor release of drugs on B16 melanoma in mice. Methods Tumor-beating mice were randomly divided into four groups： the normal saline group, the slow intra-tumor release drug group, the NCPP group and the drug combination group. Seven days later, mice were treated again and the gross tumor volume was determined. Then 2 weeks later, all the tumor-bearing mice were sacrificed to measure tumor weight. Pathologic changes of tumors were observed under light microscopy. Infiltrations of T cells were deter- mined in the splenic organs. Results Drug combination can effectively contain the tumor growth （ P 〈 0.05）. HE staining results showed that there was a large area necrotic tissue in the tumor（ P 〈 0.05）, and CD8^＋ T cells were significantly increased in the drug combination group（ P 〈 0.01）. Conclusion NCPP combined with slow intra-tumor release drugs is one of the effective management method for directly inhibiting tumor growth.