2,2′,4,4′-四溴联苯醚对鲫鱼离体肝脏组织的氧化胁迫

Oxidative Stress of Tetrabromodiphenyl Ether to the Liver of Carassius auratus. in Vitro

以鲫鱼（Carassius auratus）为试验材料,通过鲫鱼离体肝脏组织的染毒试验,研究了在离体条件下经不同浓度的2,2′,4,4′-四溴联苯醚（PBDE-47）暴露后,鲫鱼肝脏组织中总抗氧化能力（T-AOC）、丙二醛（MDA）含量、黄嘌呤氧化酶（XOD）和超氧化歧化酶（SOD）活性的动态变化。结果表明,采用浓度为0.10-10.00mg·L^-1的PBDE-47处理鲫鱼肝脏组织30min,0.10mg·L^-1浓度组鲫鱼肝脏组织中T-AOC、MDA含量、XOD和SOD活性与对照组相比无显著差异（P〉0.05）,0.56mg·L^-1以上各浓度组的XOD活性和MDA含量随PBDE-47浓度增加逐渐上升,而T-AOC和SOD活性逐渐下降,均与PBDE-47浓度呈明显的相关关系（P〈0.01）。这说明PBDE-47对鲫鱼肝脏产生了氧化损伤,具有生化毒性影响。

The present paper intends to introduce the authors＇ experimental research on the toxic effects of tetrabromodiphenyl ether（PBDE- 47 ） on the content of maleie dialdehyde（MDA）, the total anti-oxidative capacity（T-AOC ） and the activities of xanthine oxidase （XOD） and superoxide dismutase （SOD） in the liver of Carassius auratus exposed to PBDE-47. For the research purpose, we isolated the liver of the fishes to be tested and put them back to water with the concentration of PBDE-47 being, 0, 0.10, 0.56, 1.00, 1.80, 5.60 and 10.00 mg ·L^-1. The four indicators were then analyzed after a 30 min exposure in vitro at the temperature of 25 ℃. Just as the normal values of XOD and SOD would do（ 56.00-1.51 ）U· g^-1 and（ 84.72-0.58 ）U· mg^-l, the results of our experiments proved that the normal values of MDA and T-AOC tended to be separately（ 10.56-0.21 ）nmol· mg^-1 and（9.22~0.12）U·mg^-1. And the test group of 0.10 mg· L^-1 PBDE-47 had no effects on T- AOC ,MDA ,XOD and SOD in the liver after treated 30 min（P〉0.05 ）. But the other test groups, especial above 0.56 mg· L^-1, could cause the obvious changes to T-AOC, MDA, XOD and SOD （P〈0.01）. With the concentration of PBDE--47 ineresed, MDA, XOD tend to increase while T- AOC, SOD were lowering, which turned to prove T-AOC, XOD, MDA, SOD and PBDE-47 concentrations were closely related. The above results can clearly indicate that the oxidative stress and the biochemical toxicity to the liver of Carassius auratus with PBDE-47 is above 0.56 mg· L-1.