摘要
本文简要地报道了由枯草杆菌运载体pUB_(110)和大肠杆菌运载体pGEM_3构建的一个多功能穿梭载体pBE_2.该载体能在枯草杆菌和大肠杆菌中复制和表达,并具有一个强启动子和一个多酶切位点区可供广泛利用,是一个比较理想的运载体.
A multifunction shuttle vector pBE2 has been constructed by using vector pUB110 of Bacillus subtilis and vector pGEG3 of E. coli. It can be replicated and expressed in both Bacillus subtilis and E, coli. There is a strong promoter and a multiple cloning site in vector pBE2, therefore it facilitates gene manipulations in these two host strains.
出处
《江西科学》
1990年第1期17-23,共7页
Jiangxi Science
