水杨酸诱导黄瓜幼苗抗冷性的基因表达

Gene Expression of Chilling Tolerance Induced by Salicylic Acid in Cucumber Seedling

水杨酸(Salicylicacid,SA)能诱导植物提高抗生物胁迫和非生物胁迫(冷胁迫等)的能力。为研究SA对非生物胁迫下植物基因表达的影响;以黄瓜"(新优30")幼苗为试材,用SA(3mmol/L,pH6.0)溶液和蒸馏水处理后,经7℃冷胁迫48h,提取叶片总RNA并反转录cDNA,利用mRNA差异显示技术分离出12个差异表达cDNA片段,经反向斑点杂交验证其中2个(SICCL_1和SICCL_2)为高差异表达片段。序列测定和Blast分析表明,SICCL_1与甜瓜中黄瓜花叶病毒(Cucumber Mosaic Virus,CMV)相关基因AM734282有88%同源性;SICCL_2与毛柽柳中NaHCO3胁迫相关基因THL24h-106有86%同源性。由此推测,SA诱导植物抵抗生物胁迫和非生物胁迫的能力可能是通过诱导植物某些相同基因的表达来实现的。

Salicylic acid （SA） was found to improve biotic and abiotic tolerance of plants. In present paper cucumber ＂Xinyou30＂ was employed to study the effect of SA on plant gene expression and its mechanism. Under chilling stress at 7℃ cucumber seedlings were sprayed by distilled H20 and SA （3mmol/L, pH6.0）, respectively. After 48 hours total RNA was extracted from cucumber leaves and reversely transcripted into first strand cDNA. mRNA differential display was applied to analyzed SA-induced cDNA in cucumber leaves. Twelve SA-induced cDNA fragments were isolated and retracted. Two （SICCL_1 and SICCL 2）of them were affirmed by reverse dot blot to be significantly induced by SA. Nucleotide sequence analysis showed that SICCL_1 had 88% identity to AM734282 related with Cucumber Mosaic Virus （CMV） in Cucumis melo subsp. me/o; SICCL_2 had86% identity to THL24h-106 related with NaHCO3 stress in Tamarix hispida. It is postulated that SA may improve tolerance of plants via inducing overlapping genes against biotic and abiotic stresses.